Proteomics Research on the Protective Effect of Mangiferin on H9C2 Cell Injury Induced by H₂O₂

Area of Science:

• Cardiology
• Pharmacology
• Biochemistry

Background:

• Cardiovascular diseases represent a significant global health burden, causing widespread morbidity and mortality.
• Mangiferin, a natural glucosylxanthone, exhibits antioxidant and anti-inflammatory properties, suggesting cardioprotective potential.
• The precise molecular mechanisms underlying mangiferin's protection against myocardial ischemia reperfusion injury (MIRI) remain largely unexplored.

Purpose of the Study:

• To investigate the protective effects of mangiferin against MIRI using an in vitro H9C2 cell injury model.
• To elucidate the underlying molecular mechanisms of mangiferin's cardioprotection via isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics.

Main Methods:

• An in vitro model of MIRI was established using H9C2 cells subjected to hydrogen peroxide (H2O2)-induced injury.
• The protective effects of mangiferin were assessed by measuring its impact on oxidative stress and cell viability.
• iTRAQ-based proteomics was employed to identify differentially expressed proteins and explore molecular pathways affected by mangiferin.

Main Results:

• Mangiferin significantly improved the antioxidant capacity of H9C2 cells, mitigating oxidative imbalance.
• Proteomics analysis identified twenty proteins that were normalized by mangiferin pretreatment, primarily involved in glucose and fatty acid metabolism.
• Kyoto Encyclopedia of Gene and Genomes (KEGG) analysis highlighted the activation of glycolysis, the citrate cycle, and fatty acid degradation pathways.
• Western blot validation confirmed the proteomic findings for six key cardiac metabolism-related proteins.

Conclusions:

• Mangiferin demonstrates significant cardioprotective effects against MIRI by enhancing cellular antioxidant capacity.
• Mangiferin promotes cardiomyocyte survival by upregulating key metabolic pathways, including glycolysis, the citrate cycle, and fatty acid degradation.
• These findings highlight mangiferin as a potential therapeutic agent for managing MIRI and related cardiovascular conditions.