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Updated: Jan 24, 2026

Use of In Vivo Assembly for High-efficiency Plasmid Construction
Published on: February 7, 2025
The blaNDM-1-Carrying IncA/C2 Plasmid Underlies Structural Alterations and Cointegrate Formation In Vivo
Sead Hadziabdic1, Jennie Fischer1, Maria Borowiak1
1Department for Biological Safety, German Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Abstract:
In 2012, a carbapenemase-producing Salmonella enterica serovar Corvallis isolate carrying a blaNDM-1 multiresistance IncA/C2 plasmid, apart from IncHI2 and ColE-like plasmids, was detected in a wild bird in Germany. In a recent broiler chicken infection study, we observed transfer of this blaNDM-1-carrying IncA/C2 plasmid to other Enterobacteriaceae Here, we focused on the stability of this plasmid and gained insight into the type and frequency of its structural alterations after an in vivo passage in a broiler chicken infection study.
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