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Updated: Jan 24, 2026

An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
Published on: June 26, 2018
Functional cooperation of α-synuclein and VAMP2 in synaptic vesicle recycling
Jichao Sun1, Lina Wang1, Huan Bao2
1Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53705.
Abstract:
The function of α-synuclein (α-syn) has been long debated, and two seemingly divergent views have emerged. In one, α-syn binds to VAMP2, acting as a SNARE chaperone-but with no effect on neurotransmission-while another posits that α-syn attenuates neurotransmitter release by restricting synaptic vesicle mobilization and recycling. Here, we show that α-syn-VAMP2 interactions are necessary for α-syn-induced synaptic attenuation. Our data connect divergent views and suggest a unified model of α-syn function.
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