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[Passive transport of Ca2+ in a myometrium mitochondria fraction]
Na+, pH, prostaglandin F2 alpha are studied for their effect on Ca2+ transport into fractions of cow's myometrium mitochondria. Na+ does not affect a passive release of Ca2+ from mitochondria and its energy-dependent accumulation. A decrease of the incubation medium pH from 7.5 to 6.5 stimulates Ca2+ release from mitochondria and inhibits its energy-dependent pumping into them. Prostaglandin F2 alpha (10(-8)--2 X 10(-4) M) does not affect the activity of Ca2+ accumulation and release systems. A conclusion is made that the Na+-Ca2+-exchange system is absent in mitochondria of smooth muscle cells and Ca2+ release proceeds as a result of H+-Ca2+-antiport system functioning.
Na+, pH, prostaglandin F2 alpha are studied for their effect on Ca2+ transport into fractions of cow's myometrium mitochondria. Na+ does not affect a passive release of Ca2+ from mitochondria and its energy-dependent accumulation. A decrease of the incubation medium pH from 7.5 to 6.5 stimulates Ca2+ release from mitochondria and inhibits its energy-dependent pumping into them. Prostaglandin F2 alpha (10(-8)--2 X 10(-4) M) does not affect the activity of Ca2+ accumulation and release systems. A conclusion is made that the Na+-Ca2+-exchange system is absent in mitochondria of smooth muscle cells and Ca2+ release proceeds as a result of H+-Ca2+-antiport system functioning.