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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
A Strategy for Discovering Heterochiral Bioactive Peptides by Using the OB2n P Library and SPOTs Method
Hinako Udagawa1, Takato H Yoneda1, Ryo Masuda1,2
1Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University, Shinjuku, Tokyo, 169-8555, Japan.
Abstract:
d-Amino acid containing peptides are promising as drug lead compounds because of their expected higher stability in vivo. A heterochiral random peptide library called the one-bead-2n -peptide (OB2n P) library, which can display 2n peptide diastereomers per bead, has been developed. Through screening of the OB2n P library and subsequent binding assay among the peptide diastereomers synthesized in parallel by means of the SPOTs method, new heterochiral mimotopes for the anti-β-endorphin monoclonal antibody have been obtained. One mimotope was a new ligand for the μ-opioid receptor. The screening strategy enabled d-amino acid containing drug leads to be obtained efficiently by expanding searchable chemical space without increasing the experimental scale.
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