Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

14.8K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.8K
Antibiotic Selection00:57

Antibiotic Selection

59.6K
Overview
59.6K
What is Natural Selection?01:32

What is Natural Selection?

126.4K
Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
126.4K
Energy-releasing Steps of Glycolysis01:28

Energy-releasing Steps of Glycolysis

146.5K
Glycolysis is divided into two phases based on whether energy is utilized or released. While the first phase consumes ATP, the second phase produces energy in the form of ATP and NADH. The energy is released over a sequence of reactions that turns G3P into pyruvate. The energy-releasing phase—steps 6-10 of glycolysis—occurs twice, once for each of the two 3-carbon sugars produced during steps 1-5 of the first phase.
The first energy-releasing step—the 6th step of glycolysis...
146.5K
Hybridoma Technology01:31

Hybridoma Technology

17.3K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
17.3K
Types of Selection01:46

Types of Selection

44.2K
Natural selection influences the frequencies of particular alleles and phenotypes within populations in several different ways. Primarily, natural selection can be directional, stabilizing, or disruptive. Directional selection favors one extreme trait and shifts the population towards that phenotype while selecting against individuals displaying alternate traits. Stabilizing selection favors an intermediate trait with a narrow range of variation. Deviation from the optimal phenotype towards an...
44.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same author

A microfluidic platform for whole-membrane integrity profiling in live neuronal cells.

Microsystems & nanoengineering·2026
Same author

Inverted-T Mastopexy With Glandular Treatment and Breast Augmentation: Outcomes and Stabilization Approach.

Plastic and reconstructive surgery. Global open·2026
Same author

MarrowCellDLD: a microfluidic method for label-free retrieval of fragile bone marrow-derived cells.

Scientific reports·2023
Same author

Prediction of Dispersion Rate of Airborne Nanoparticles in a Gas-Liquid Dual-Microchannel Separated by a Porous Membrane: A Numerical Study.

Micromachines·2022
Same author

Ionic transistor using ion exchange membranes.

Lab on a chip·2022
Same journal

Optimisation of Electrokinetic Extraction System: Colourimetric Determination of Copper (II) in Sand Using Polymer Inclusion Membrane.

Electrophoresis·2026
Same journal

Novel Phloroglucinol Derivatives as Neuraminidase Inhibitors Identified From Humulus lupulus L. Extract by At-Line Nanofractionation Platform.

Electrophoresis·2026
Same journal

Protein-Based High-Performance Liquid Chromatography and Cyclodextrin-Capillary Electrokinetic Chromatography for the Chiral Separation of Azoles.

Electrophoresis·2026
Same journal

Dynamics of Heparin Translocations Through Solid-State Nanopores.

Electrophoresis·2026
Same journal

Production of Protein Hydrolysates and Bioactive Peptides From Lablab purpureus and Macrotyloma uniflorum via Optimized Extraction and Proteolysis Protocols.

Electrophoresis·2026
Same journal

CMOS Electrokinetic Systems and Fabrication Approaches for On-CMOS 3D Electrodes.

Electrophoresis·2026
See all related articles

Related Experiment Video

Updated: Jan 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K

On-chip technology for single-cell arraying, electrorotation-based analysis and selective release.

Kevin Keim1, Mohamed Z Rashed1, Samuel C Kilchenmann1

  • 1Laboratory of Life Sciences Electronics, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.

Electrophoresis
|May 22, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method for label-free single-cell biophysical analysis using electrokinetic forces. It enables simultaneous electrorotation analysis of multiple cells, revealing their dielectric properties.

Keywords:
Dielectrophoretic trappingElectrorotationSingle-cell analysisSingle-cell arraySingle-cell release

More Related Videos

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
08:32

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

9.5K
Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

608

Related Experiment Videos

Last Updated: Jan 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.5K
Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates
08:32

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

9.5K
Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

608

Area of Science:

  • Biophysics
  • Cell Biology
  • Microfluidics

Background:

  • Accurate single-cell analysis is crucial for understanding cellular heterogeneity.
  • Existing methods for cell biophysical analysis can be complex and time-consuming.
  • Label-free techniques are desirable to avoid altering cell properties.

Purpose of the Study:

  • To develop a label-free method for simultaneous biophysical analysis of multiple single cells.
  • To utilize electrokinetic forces for precise cell trapping, manipulation, and dielectric property measurement.
  • To demonstrate the system's capability across various cell types and sizes.

Main Methods:

  • Fabrication of microfluidic chips with 3D pillar electrodes for electrokinetic cell manipulation.
  • Application of dielectrophoresis (DEP) and electrorotation for cell trapping and analysis.
  • Measurement of cell rotation speed versus electric field frequency to generate electrorotation spectra.
  • Simultaneous analysis of multiple cells in individually addressable micro-cages.

Main Results:

  • Successful simultaneous electrorotation analysis of multiple single cells.
  • Accurate dielectric property measurements for Henrietta Lacks, HEK 293, and T lymphocytes, consistent with prior research.
  • Determination of membrane capacitance for M17 neuroblastoma cells (7.49 ± 0.39 mF/m²).
  • Demonstration of selective cell trapping and release.

Conclusions:

  • The developed microfluidic system enables efficient, label-free, multi-cell biophysical analysis.
  • Electrorotation spectroscopy provides valuable insights into cell dielectric properties.
  • The platform is versatile for studying diverse cell types and sizes, advancing cell biology research.