GABAergic and glutamatergic cells in the inferior colliculus dynamically express the GABA_AR γ₁ subunit during aging

Age-related hearing loss may result, in part, from declining levels of γ-amino butyric acid (GABA) in the aging inferior colliculus (IC). An upregulation of the GABA_AR γ₁ subunit, which has been shown to increase sensitivity to GABA, occurs in the aging IC. We sought to determine whether the upregulation of the GABA_AR γ₁ subunit was specific to GABAergic or glutamatergic IC cells. We used immunohistochemistry for glutamic acid decarboxylase and the GABA_AR γ₁ subunit at 4 age groups in the IC of Fisher Brown Norway rats. The percentage of somas that expressed the γ₁ subunit and the number of subunits on each soma were quantified. Our results show that GABAergic and glutamatergic IC cells increasingly expressed the γ₁ subunit from young age until expression peaked during middle age. At old age (∼77% of life span), the number of GABA_AR γ₁ subunits per cell sharply decreased for both cell types. These results, along with previous studies, suggest inhibitory and excitatory IC circuits may express the GABA_AR γ₁ subunit in response to the age-related decline of available GABA.