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LncRNA PACER is down-regulated in osteoarthritis and regulates chondrocyte apoptosis and lncRNA HOTAIR expression

Mingwei Jiang1, Jie Liu2, Tao Luo1

  • 1Department of orthopaedics, Shanghai Public Health Clinical Center, Shanghai City 201508, P.R. China.

Bioscience Reports
|May 23, 2019
PubMed

Insights

Long non-coding RNA PACER is down-regulated in osteoarthritis (OA) and inhibits chondrocyte apoptosis by reducing HOTAIR. This finding offers new insights into OA pathogenesis and potential therapeutic targets.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Chondrocyte inflammation is a key factor in osteoarthritis (OA) development.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cellular processes and disease.
  • PACER and HOTAIR are lncRNAs with potential involvement in OA pathogenesis.

Purpose of the Study:

  • To investigate the role of lncRNA PACER in osteoarthritis (OA).
  • To determine the relationship between lncRNA PACER and lncRNA HOTAIR in OA patients.
  • To elucidate the mechanism by which PACER influences chondrocyte apoptosis in OA.

Main Methods:

  • Analysis of plasma PACER and HOTAIR levels in OA patients and healthy controls.
  • Correlation analysis between PACER and HOTAIR expression.
  • Experimental manipulation of PACER and HOTAIR expression in chondrocytes.
  • Assessment of chondrocyte apoptosis following lncRNA manipulation.

Main Results:

  • Plasma PACER levels were down-regulated, while HOTAIR levels were up-regulated in OA patients.
  • Altered PACER and HOTAIR levels distinguished OA patients from controls.
  • PACER and HOTAIR showed an inverse correlation in both groups.
  • PACER overexpression down-regulated HOTAIR and inhibited chondrocyte apoptosis.
  • HOTAIR overexpression promoted chondrocyte apoptosis and attenuated PACER's effects.

Conclusions:

  • lncRNA PACER is significantly down-regulated in osteoarthritis.
  • PACER plays a protective role by inhibiting chondrocyte apoptosis.
  • PACER exerts its function, at least in part, by down-regulating HOTAIR.
  • PACER represents a potential diagnostic biomarker and therapeutic target for OA.

Keywords:
chondrocytelncRNA HOTAIRlncRNA PACERosteoarthritis

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