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The Pipeline Repertoire for Ig-Seq Analysis
Laura López-Santibáñez-Jácome1,2, S Eréndira Avendaño-Vázquez1, Carlos Fabián Flores-Jasso1
1Consorcio de Metabolismo de RNA, Instituto Nacional de Medicina Genómica, Mexico City, Mexico.
Frontiers in Immunology
|May 23, 2019
Summary
High-throughput sequencing of immunoglobulin genes (Ig-Seq) offers insights into antibody repertoires. This review guides researchers through diverse computational tools for immune repertoire analysis, aiding experimental design and data interpretation.
Area of Science:
- Immunology
- Bioinformatics
- Genomics
Background:
- High-throughput sequencing of immunoglobulin genes (Ig-Seq) has revolutionized the study of antibody repertoires.
- Characterizing immune repertoires is crucial for understanding adaptive immunity.
- A growing number of computational tools exist for immune repertoire analysis.
Purpose of the Study:
- To provide a practical and organized overview of current computational tools for antibody repertoire characterization.
- To highlight the unique features and differences of various analysis pipelines.
- To assist researchers in selecting appropriate tools and designing effective immune repertoire studies.
Main Methods:
- Review of existing computational tools for immune repertoire analysis.
- Comparative analysis of tool functionalities and capabilities.
- Focus on practical aspects for laboratory implementation.
Main Results:
- Identification of a diverse landscape of computational tools for Ig-Seq data analysis.
- Elucidation of the distinct strengths and rationales behind different analysis pipelines.
- Guidance on strategic approaches for experimental design and computational workflows.
Conclusions:
- Understanding the nuances of different computational tools is essential for robust immune repertoire analysis.
- This review empowers researchers to make informed decisions for their Ig-Seq studies.
- Facilitating wider adoption and advancement of immune repertoire research.
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