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Methods to Study Changes in Inherent Protein Aggregation with Age in Caenorhabditis elegans
Published on: November 26, 2017
Viscosity of mixtures of protein aggregates with different sizes and morphologies
Walailuk Inthavong1, Christophe Chassenieux, Taco Nicolai
1Institut des Molécules et Matériaux du Mans, IMMM - UMR 6283 CNRS, Polymères, Colloïdes et Interfaces Le Mans Université, Avenue Olivier Messiaen, 72085 LE MANS CEDEX 9, France. christophe.chassenieux@univ-lemans.fr.
Abstract:
Protein aggregates were generated by thermal denaturation of whey protein isolates. Depending on the heating conditions, fractal aggregates of various sizes or microgels were obtained. The osmotic compressibility and correlation length of mixtures of fractal aggregates of different sizes were found to be close to the weighted averages of the individual components at the same concentration. The viscosity of these mixtures can be described by a logarithmic mixing law using the weight fraction and the viscosity of the individual components. The same mixing law describes the behavior of mixtures of fractal aggregates and microgels. The effect of the type of protein was investigated by mixing fractal aggregates formed by whey and soy protein isolates. It is suggested that the viscosity of the mixtures is determined by the cooperative movement over length scales much larger than the size of the aggregates.
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