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MiR-1294 acts as a tumor suppressor in clear cell renal cell carcinoma through targeting HOXA6

W Pan1, L-J Pang, H-L Cai

  • 1Department of Urinary Surgery, RenMin Hospital of Huangpi District, the Third Hospital of Jianghan University, Huangpi District, Wuhan, P. R. China. panwei_wh@163.com.

European Review for Medical and Pharmacological Sciences
|May 23, 2019
PubMed
Abstract

Insights

MicroRNA-1294 (miR-1294) acts as a tumor suppressor in clear cell renal cell carcinoma (ccRCC). Downregulation of miR-1294 correlates with poor survival, and its restoration inhibits ccRCC progression by targeting HOXA6.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cancer, primarily clear cell renal cell carcinoma (ccRCC), accounts for 3% of human cancers.
  • MicroRNAs (miRNAs) are implicated in cancer development and progression.
  • Understanding specific miRNA roles, like miR-1294, is crucial for ccRCC research.

Purpose of the Study:

  • To investigate the expression of miR-1294 in ccRCC.
  • To elucidate the mechanisms of miR-1294 in ccRCC progression.
  • To identify potential therapeutic targets related to miR-1294.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for miR-1294 expression analysis.
  • Kaplan-Meier plotter for survival analysis.
  • Cell counting kit-8, colony formation, and transwell invasion assays for cellular functions.
  • Luciferase reporter and Western blot assays to validate miR-1294 and HOXA6 interaction.

Main Results:

  • miR-1294 was found to be downregulated in ccRCC cell lines.
  • Lower miR-1294 expression correlated with poorer overall survival in ccRCC patients.
  • Overexpression of miR-1294 inhibited ccRCC cell proliferation, colony growth, and invasion.
  • Homeobox A6 (HOXA6) was identified as a direct target of miR-1294 and negatively regulated by it.

Conclusions:

  • miR-1294 functions as a tumor suppressor in ccRCC.
  • Restoring miR-1294 levels can inhibit ccRCC cell proliferation, colony formation, and invasion.
  • The tumor-suppressive role of miR-1294 is partially mediated through its regulation of HOXA6.

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