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Updated: Jan 24, 2026

Murine Colitis Modeling using Dextran Sulfate Sodium DSS
08:39

Murine Colitis Modeling using Dextran Sulfate Sodium DSS

Published on: January 19, 2010

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Administration of live, but not inactivated, Faecalibacterium prausnitzii has a preventive effect on dextran sodium

Yujiro Kawade1, Misaki Sakai1, Mariko Okamori1

  • 1Central Research Laboratories, Nichinichi Pharmaceutical Co., Ltd., Iga, Mie 518‑1417, Japan.

Molecular Medicine Reports
|May 23, 2019
PubMed

Insights

Live Faecalibacterium prausnitzii, a key gut microbe, shows promise in preventing and treating colitis. Administration of live F. prausnitzii significantly improved symptoms and reduced inflammation in a mouse model of inflammatory bowel disease.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Immunology

Background:

  • Faecalibacterium prausnitzii is a dominant human gut bacterium.
  • This microbe is implicated in the pathogenesis of inflammatory bowel diseases (IBD).

Purpose of the Study:

  • To investigate the protective effects of live and inactivated Faecalibacterium prausnitzii against dextran sodium sulfate (DSS)-induced colitis in mice.
  • To evaluate both preventive and therapeutic administration of F. prausnitzii.

Main Methods:

  • A dextran sodium sulfate (DSS)-induced colitis model was established in BALB/c mice.
  • Mice received live or inactivated F. prausnitzii pre- and post-DSS administration.
  • Analysis included assessment of colitis symptoms, mucosal cytokine expression (RT-qPCR), and colon histology.

Main Results:

  • Live F. prausnitzii significantly ameliorated DSS-induced colitis symptoms, including weight loss, diarrhea, and colon shortening.
  • While not altering proinflammatory cytokines, live F. prausnitzii administration markedly reduced mucosal cytokine expression.
  • Therapeutic administration of live F. prausnitzii also improved histological scores in mice with established colitis.

Conclusions:

  • Preventive administration of live, but not inactivated, F. prausnitzii confers protection against experimental colitis.
  • Live F. prausnitzii demonstrates potential as a therapeutic agent for inflammatory bowel diseases.

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