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Hyperpolarising Pyruvate through Signal Amplification by Reversible Exchange (SABRE)
Wissam Iali1, Soumya S Roy1,2, Ben J Tickner1
1Centre for Hyperpolarisation in Magnetic Resonance (CHyM), Department of Chemistry, University of York, Heslington, York, YO10 5NY, UK.
Angewandte Chemie (International Ed. in English)
|May 23, 2019
Summary
Signal amplification by reversible exchange (SABRE) hyperpolarization creates strong 13C pyruvate signals rapidly. This novel method enhances magnetic resonance imaging for cellular metabolism studies and chemical reactivity investigations.
Area of Science:
- Magnetic Resonance
- Metabolic Imaging
- Catalysis
Background:
- Hyperpolarization techniques significantly enhance magnetic resonance (MR) sensitivity.
- This enables disease tracking and cellular metabolism interrogation via MR.
- Pyruvate is a key metabolic substrate for MR studies.
Purpose of the Study:
- To develop a novel hyperpolarization method for 13C pyruvate using Signal Amplification By Reversible Exchange (SABRE).
- To demonstrate rapid and strong signal enhancements for 13C pyruvate.
- To explore the potential for observing metabolic pathways and chemical reactivity.
Main Methods:
- Utilized Signal Amplification By Reversible Exchange (SABRE) for hyperpolarization.
- Synthesized a novel iridium-based catalyst: [Ir(H)2(η2-pyruvate)(DMSO)(IMes)].
- Applied SABRE to achieve hyperpolarization of [1-13C]-, [2-13C]-, and [1,2-13C2]-pyruvate.
Main Results:
- Achieved strong 13C pyruvate signal enhancements in seconds using SABRE.
- Successfully generated hyperpolarized [1-13C]- and [2-13C]pyruvate.
- Observed a long-lived singlet state in hyperpolarized [1,2-13C2]pyruvate for up to five minutes post-polarization.
Conclusions:
- SABRE provides a rapid and effective method for hyperpolarizing 13C pyruvate.
- This technique offers significant sensitivity gains for MR-based metabolic studies.
- The observed long-lived state in [1,2-13C2]pyruvate opens avenues for advanced chemical reactivity studies.
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