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Decrease of spermatozoa with an unbalanced chromosome content after cell sorting in men carrying a structural

S El Fekih1,2, C Tous3, N Gueganic1

  • 1Laboratoire d'histologie, Embryologie et Cytogénétique, INSERM U1078, Faculté de Médecine et des Sciences de la Santé, Université de Bretagne Occidentale, Brest, France.

Andrology
|May 23, 2019
PubMed
Summary
This summary is machine-generated.

Magnetic-activated cell separation effectively selects healthier sperm in men with chromosomal abnormalities. This technique reduces DNA fragmentation and chromosomal errors, improving sperm quality for assisted reproduction.

Keywords:
DNA fragmentationchromosomally unbalancedmagnetic-activated cell separationspermatozoa

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Area of Science:

  • Reproductive Biology
  • Genetics
  • Cell Biology

Background:

  • Men with chromosomal abnormalities often have higher DNA fragmentation in unbalanced sperm.
  • This may be linked to abortive apoptosis, a known issue in infertile men.

Purpose of the Study:

  • To evaluate magnetic-activated cell separation (MACS) for selecting sperm with reduced DNA fragmentation and chromosomal errors.
  • Focus on men with structural chromosomal abnormalities.

Main Methods:

  • Sperm from ten males with chromosomal rearrangements were separated using MACS (annexin V (-) and (+) fractions).
  • Analyzed meiotic segregation, phosphatidylserine externalization, and DNA fragmentation using fluorescence in situ hybridization, annexin V staining, and TUNEL assay.

Main Results:

  • MACS significantly reduced phosphatidylserine externalization and DNA fragmentation in the annexin V (-) fraction.
  • The annexin V (-) fraction showed a significant increase in normal/balanced spermatozoa.
  • Conversely, the annexin V (+) fraction had higher DNA fragmentation and fewer normal/balanced sperm.

Conclusions:

  • MACS is a promising tool for selecting healthier sperm in men with chromosomal abnormalities.
  • This method can decrease sperm with DNA fragmentation and chromosomal errors for assisted reproductive technologies like ICSI.