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Circular RNA 0001785 regulates the pathogenesis of osteosarcoma as a ceRNA by sponging miR-1200 to upregulate HOXB2

Shenglong Li1, Yi Pei1, Wei Wang1

  • 1a Department of Bone and Soft Tissue Tumor Surgery , Cancer Hospital of China Medical University, Liaoning Cancer Hospital & Institute , Shenyang , Liaoning Province , China.

Cell Cycle (Georgetown, Tex.)
|May 23, 2019
PubMed

Insights

Circular RNAs (circRNAs) show promise as cancer targets. This study found circ-0001785 promotes osteosarcoma by regulating miR-1200 and HOXB2, impacting cell growth and apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Osteosarcoma is a prevalent bone cancer in children and adolescents.
  • Circular RNAs (circRNAs) are emerging as significant therapeutic targets in various tumors.
  • Understanding the role of specific circRNAs in osteosarcoma progression is crucial.

Purpose of the Study:

  • To investigate the expression pattern and biological function of circ-0001785 in osteosarcoma.
  • To elucidate the regulatory mechanism of circ-0001785, including its interaction with miR-1200 and HOXB2.
  • To explore the impact of the circ-0001785/miR-1200/HOXB2 axis on osteosarcoma cell behavior.

Main Methods:

  • CircRNA microarray analysis to identify differentially expressed circRNAs.
  • Quantitative real-time PCR to determine expression levels of circ-0001785 and miR-1200.
  • Bioinformatics analysis and dual-luciferase reporter gene assay to confirm binding interactions.
  • Cell proliferation (EdU assay) and apoptosis (flow cytometry) assays to assess functional effects.
  • Western blot analysis to investigate signaling pathway regulation.

Main Results:

  • Circ-0001785 was found to be highly expressed in osteosarcoma cell lines.
  • Knockdown of circ-0001785 inhibited osteosarcoma cell proliferation and induced apoptosis.
  • Circ-0001785 acts as a molecular sponge for miR-1200, leading to the upregulation of its target gene, HOXB2.
  • The circ-0001785/miR-1200/HOXB2 axis influences the PI3K/Akt and Bcl-2 family signaling pathways.

Conclusions:

  • Circ-0001785 plays a significant role in the pathogenesis of osteosarcoma.
  • The mechanism involves sponging miR-1200 to upregulate HOXB2 expression.
  • Circ-0001785 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
OsteosarcomaapoptosiscircRNAcompeting endogenous RNAs (ceRNA)proliferation

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