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Updated: Jan 24, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
Adipogenesis associated Mth938 domain containing (AAMDC) protein expression is regulated by alternative
Rong Xiao1, Chengping Li1, Chao Wang1
1College of Life Science, Liaocheng University, China.
Abstract:
The post-transcriptional events regulating expression of the adipogenesis associated Mth938 domain containing (AAMDC) protein are poorly understood. Here, we find that AAMDC expresses three isoforms due to alternative polyadenylation (APA) and alternative splicing (AS). Luciferase assay revealed that expression of the two isoforms with short and long 3'UTRs is differentially controlled. Further analyses showed that the short isoform displays higher translation efficiency and that miR-2428/664a reduces expression of the long isoform, indicating that APA-mediated shortening of the AAMDC 3'UTR renders the short isoform insusceptible to miRNA-mediated suppression due to loss of the binding sites for miR-2428/664a. Finally, we demonstrate that the short AAMDC isoform promotes bovine preadipocyte differentiation. Collectively, our findings indicate that AAMDC is post-transcriptionally regulated through APA and microRNAs.
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