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Updated: Jan 24, 2026

Overlapping Peptide Library to Map Qa-1 Epitopes in a Protein
Published on: December 20, 2017
Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides
Chandra Sekhar Bokka1, Ganesh Kumar Veeramachaneni2, V B S C Thunuguntla3
1Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Green fields, Vaddeswaram, Guntur Dist., Andhra Pradesh 522 502, India. urschandu18@gmail.com.
Background And Objectives:
Nearly 20-30% of the world's population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized.
Materials And Methods:
To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice.
Results:
Peptide PF3 and PF4 displayed high docking G-scores (-9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when compared to other peptides and controls, signifying a strong, Th2-based response. Concurrently, the Th1 pathway was inhibited by low levels of cytokine IL2, IFN-γ, and IL-10 justifying the role of PF3 in allergenic IgE response.
Conclusions:
Based on the results of overlapping peptides PF3 and PF4, the N-terminal part of the PF3 peptide (TGQALVI) plays a crucial role in allergenic response of Sorghum profilin.
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