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PARP1 Inhibition as a Novel Therapeutic Target for Keloid Disease

Tae Hwan Park1, Chan Woo Kim1, Jin Sik Choi1

  • 1Department of Plastic and Reconstructive Surgery, CHA Bundang Medical Center, CHA University School of Medicine, Seongnam, Republic of Korea.

Advances in Wound Care
|May 24, 2019
PubMed

Insights

Poly(ADP-ribose) polymerase 1 (PARP1) inhibition shows promise for keloid treatment. The PARP1 inhibitor rucaparib reduced keloid fibroblast proliferation and migration, suggesting PARP1 as a therapeutic target.

Area of Science:

  • Dermatology
  • Oncology
  • Molecular Biology

Background:

  • Poly(ADP-ribose) polymerase 1 (PARP1) inhibition demonstrates protective effects in fibrotic diseases.
  • The therapeutic potential of PARP1 inhibition in keloid treatment remains unexplored.

Purpose of the Study:

  • To evaluate the therapeutic efficacy of the PARP1 inhibitor rucaparib in keloids.
  • To investigate PARP1 and smad3 protein expression in keloid tissues.

Main Methods:

  • Western blotting was used to assess PARP1 and smad3 protein levels in keloid and control tissues.
  • In vitro assays (MTT and migration assays) evaluated rucaparib's effects on keloid fibroblasts.
  • A patient-derived keloid xenograft murine model was used to assess in vivo efficacy.

Main Results:

  • PARP1 and smad3 protein expression were significantly elevated in keloid tissues.
  • Rucaparib suppressed keloid fibroblast proliferation and migration.
  • Rucaparib treatment reduced fibrosis markers and xenograft keloid size.

Conclusions:

  • PARP1 is a potential novel therapeutic target for keloid disease.
  • Rucaparib demonstrates promise as a therapeutic agent for treating keloids.
Keywords:
PARP1fibroblastkeloidrucaparib

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