Advances in Using Hansenula polymorpha as Chassis for Recombinant Protein Production

Area of Science:

• Biotechnology
• Microbiology
• Molecular Biology

Background:

• Hansenula polymorpha is a non-conventional methylotrophic yeast utilized for recombinant protein production for over 30 years.
• Previous reviews on H. polymorpha as a cell factory are outdated, necessitating an updated summary of its applications.
• The yeast serves as a host for producing diverse biomolecules, including enzymes, vaccines, and biopharmaceuticals.

Purpose of the Study:

• To review the advancements in using Hansenula polymorpha as a host for recombinant protein production over the last decade.
• To discuss the production of specific chemicals and virus-like particles using H. polymorpha.
• To provide a comprehensive guideline for utilizing H. polymorpha as an expression platform.

Main Methods:

• Summarizing recent literature on H. polymorpha's use in recombinant protein production.
• Highlighting genetic manipulation techniques and bioreactor cultivation strategies.
• Discussing the application of novel genomic editing tools like CRISPR/Cas9.

Main Results:

• H. polymorpha continues to be a valuable host for producing recombinant proteins, chemicals, and virus-like particles.
• Recent advancements include improved genetic manipulation techniques and cultivation strategies.
• CRISPR/Cas9 has been successfully established for genomic editing in H. polymorpha.
• Case studies on producing human Parathyroid Hormone (PTH) and Staphylokinase (SAK) demonstrate process establishment.

Conclusions:

• Hansenula polymorpha remains a highly effective and versatile expression platform for biopharmaceutical production.
• The review provides an updated analysis of genetic aspects and fermentation protocols for H. polymorpha.
• Encourages further research and production of novel biomolecules using this yeast host.