A Core-Shell Structured Immobilized Lipase and Its Application in High-Temperature Reactions

Area of Science:

• Biocatalysis
• Materials Science
• Chemical Engineering

Background:

• Enzyme immobilization is crucial for industrial biocatalysis.
• Developing cost-effective and efficient carriers remains a challenge.
• Lipases are versatile biocatalysts with broad applications.

Purpose of the Study:

• To develop a core-shell immobilized lipase using a bamboo-based carrier.
• To optimize immobilization conditions for enhanced lipase performance.
• To evaluate the stability and reusability of the immobilized lipase in wax ester synthesis.

Main Methods:

• Bamboo powder was pretreated using alkali treatment.
• Core-shell immobilization was achieved using sodium alginate and calcium chloride.
• Response surface methodology was employed to optimize immobilization parameters.
• Scanning Electron Microscopy (SEM) and Brunauer–Emmett–Teller (BET) analyses were performed.

Main Results:

• Alkali pretreatment of bamboo enhanced surface roughness and microporosity, improving lipase adsorption.
• Optimal immobilization conditions were determined: 2 wt% NaOH, 1.2 wt% sodium alginate, 0.69 wt% CaCl2, 100 min calcification.
• The immobilized lipase exhibited high protein adsorption, excellent thermal stability, and maintained 80% esterification yield after 17 batches.
• The core-shell immobilized lipase demonstrated significant reusability and stability in wax ester synthesis.

Conclusions:

• The developed core-shell immobilized lipase on a bamboo carrier is effective for biocatalytic applications.
• The immobilization strategy offers a promising approach for industrial enzyme applications.
• This method provides a cost-effective and sustainable alternative for enzyme immobilization.