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Related Experiment Video

Updated: Jan 24, 2026

Yeast Colony Embedding Method
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Gonococcal Colony Typing.

John E Heckels1

  • 1Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK. jeh@soton.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2019
PubMed
Summary

Gonococcal colony typing, a blend of science and art, is crucial for understanding Neisseria gonorrhoeae virulence and variation. This method remains essential for current research and monitoring bacterial experiments.

Area of Science:

  • Microbiology
  • Bacteriology
  • Pathogenesis Studies

Background:

  • Gonococcal colony typing has been fundamental in identifying key virulence factors and understanding the rapid phase and antigenic variation in Neisseria gonorrhoeae.
  • This foundational technique underpins modern molecular studies of gonococcal pathogenesis.

Purpose of the Study:

  • To describe the methods for performing gonococcal colony typing.
  • To present techniques for optimizing the colony typing process.

Main Methods:

  • Detailed description of gonococcal colony typing procedures.
  • Explanation of optimization techniques for improved accuracy and efficiency.

Main Results:

  • Colony typing enables identification of crucial virulence antigens.
Keywords:
Colony typingNeisseria gonorrhoeaeOpa proteinPili

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  • Demonstrates the capacity of Neisseria gonorrhoeae for rapid phase and antigenic variation.
  • Highlights the continued essential role of colony typing in biological experiments.

    • Conclusions:

    • Gonococcal colony typing is an indispensable technique in microbiology.
    • It remains vital for ongoing research in gonococcal pathogenesis and for monitoring experimental outcomes.