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Related Experiment Video

Updated: Jan 24, 2026

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Basic Methods for Examining Neisseria gonorrhoeae Interactions with Host Cells In Vitro.

Hannia Liliana Almonacid-Mendoza1, Myron Christodoulides2

  • 1Molecular Microbiology Group, Academic Unit of Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2019
PubMed
Summary

This study explores how Neisseria gonorrhoeae, the cause of gonorrhea, interacts with human cells. Protocols are provided to examine these basic bacterial-host cell interactions for better understanding of the infection process.

Keywords:
AssociationCell cultureCell deathCytokine productionInvasionMicroscopy

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Area of Science:

  • Microbiology
  • Immunology
  • Pathogenesis

Background:

  • Neisseria gonorrhoeae is an obligate human pathogen causing gonorrhea.
  • It primarily colonizes mucosal epithelia but can disseminate, leading to serious conditions like disseminated gonococcal infection.
  • Host response involves inflammation and interactions with various immune cells.

Purpose of the Study:

  • To provide protocols for examining basic interactions between Neisseria gonorrhoeae and human cells.
  • To facilitate understanding of gonococcal pathogenesis and host-pathogen interactions.
  • To aid in identifying bacterial factors and host responses involved in infection.

Main Methods:

  • Utilizing ex vivo models with host tissues and eukaryotic cell monocultures.
  • Examining interactions between Neisseria gonorrhoeae and a representative human cell line.
  • Employing established protocols to study bacterial adhesins, invasins, and host cell receptors.

Main Results:

  • Ex vivo models have successfully identified bacterial adhesins and invasins.
  • These models have elucidated the roles of specific cell surface receptors in gonococcal pathogenesis.
  • Understanding of virulence mechanisms and immune responses has been advanced.

Conclusions:

  • Protocols for studying Neisseria gonorrhoeae interactions with human cells are essential for research.
  • These methods contribute to understanding gonorrhea pathogenesis and host immune responses.
  • Further research using these models can identify novel therapeutic targets.