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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
Published on: June 30, 2016
Use of Human Monocyte-Derived Macrophages to Study Neisseria gonorrhoeae Infection
Jimena Gatica1, Paula I Rodas2, Alejandro Escobar3
1Instituto de Investigación en Ciencias Odontológicas, Facultad de Odontología, Universidad de Chile, Santiago, Chile.
Abstract:
Macrophages are critical cells in the innate immune response to microorganisms sensed in the tissues. During infections, the interaction between pathogens and macrophages leads to a macrophage response that includes cytokine production, antigen processing and presentation in the context of MHC molecules, expression of T cell costimulatory molecules and recruitment of innate defense effectors, which results in clearance of infection. However, Neisseria gonorrhoeae can suppress the protective immune response at this level, avoiding its detection and elimination. Studies addressed to develop the interactions between macrophages and Neisseria gonorrhoeae allow us to find potential targets to be exploited with vaccines and therapeutic drugs. In this chapter, we describe protocols to generate human monocyte-derived macrophages and assess their response to infection with Neisseria gonorrhoeae.
Insights
This study details methods for generating human monocyte-derived macrophages to investigate their response to Neisseria gonorrhoeae. Understanding this interaction is key to developing new treatments for gonorrhea infections.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Macrophages are crucial for innate immunity against pathogens.
- Neisseria gonorrhoeae evades immune detection by suppressing macrophage responses.
- Targeting macrophage-pathogen interactions offers therapeutic and vaccine potential.
Purpose of the Study:
- To outline protocols for generating human monocyte-derived macrophages.
- To assess the response of these macrophages upon Neisseria gonorrhoeae infection.
Main Methods:
- Generation of human monocyte-derived macrophages from peripheral blood monocytes.
- Infection of macrophages with Neisseria gonorrhoeae.
- Assessment of macrophage activation and immune response markers.
Main Results:
- Protocols established for reproducible generation of human macrophages.
- Demonstrated methods to analyze macrophage-pathogen interactions in vitro.
- Provided a framework for studying Neisseria gonorrhoeae immune evasion strategies.
Conclusions:
- The described protocols facilitate research into macrophage-Neisseria gonorrhoeae interactions.
- This work supports the development of novel vaccines and therapeutics against gonorrhea.
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