Related Experiment Video
Updated: Jan 24, 2026
10:12
Neisseria meningitidis Infection of Induced Pluripotent Stem-Cell Derived Brain Endothelial Cells
Published on: July 14, 2020
2.7K
Female Mouse Model of Neisseria gonorrhoeae Infection
Erica L Raterman1, Ann E Jerse2
1Uniformed Services, University of the Health Sciences, Bethesda, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2019
Summary
The estradiol-treated mouse model aids gonorrhea research by studying immune evasion and antibiotic resistance. This model helps understand Neisseria gonorrhoeae infection despite limitations in mimicking human reproductive cycles.
Area of Science:
- Microbiology
- Immunology
- Infectious Diseases
Background:
- Mouse models are crucial for studying infectious diseases and developing treatments.
- The estradiol-treated mouse model is valuable for Neisseria gonorrhoeae genital tract infection research.
- This model helps investigate gonococcal immune evasion and adaptation to host factors.
Purpose of the Study:
- To detail methods for experimental Neisseria gonorrhoeae infection in mice.
- To enable quantitative culture of vaginal swabs for infection studies.
- To monitor the host innate immune response during gonorrhea infection.
Main Methods:
- Preparation of mice for experimental Neisseria gonorrhoeae infection.
- Inoculation of mice and quantitative culture of vaginal swabs.
- Monitoring the host innate immune response to infection.
Main Results:
- The estradiol-treated mouse model facilitates studies on gonococcal immune evasion.
- This model is useful for assessing antibiotic resistance mutations' impact on gonococcal fitness.
- It aids in the search for new antibiotics against resistant Neisseria gonorrhoeae strains.
Conclusions:
- The described methods support the use of estradiol-treated mice for gonorrhea research.
- Improvements in animal modeling enhance the study of Neisseria gonorrhoeae infection.
- This model system is vital for understanding host-pathogen interactions and developing new therapies.
Keywords:
ChemokinesCytokinesEstradiolEstrous cycleFemale mouse model of gonorrhea infectionNeisseria gonorrhoeaePolymorphonuclear leukocytesMore Related Videos
07:26Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands
Published on: March 4, 2020
6.0K
08:04Quantitative Examination of Antibiotic Susceptibility of Neisseria gonorrhoeae Aggregates Using ATP-utilization Commercial Assays and Live/Dead Staining
Published on: February 8, 2019
9.1K
Related Concept Videos
01:28Infertility in Females
3.7K
Female infertility is defined as the inability to conceive after a year of regular, unprotected intercourse and affects about 10–15% of couples worldwide. The primary cause of female infertility is ovulatory disorders, which hinder the release of eggs. These disorders can be classified as hypothalamic amenorrhea, polycystic ovarian syndrome (PCOS), premature ovarian failure, and hyperprolactinemic anovulation disorders.
Endometriosis, a condition characterized by abnormal growth of...
Endometriosis, a condition characterized by abnormal growth of...
3.7K
01:15External Female Genitals
31.6K
The vulva encompasses the external structures of the female reproductive system. At the forefront is the monpubis, a cushion of fatty tissue atop the pubic bone. Once puberty sets in, this area typically grows hair. Extending from just behind the mons pubis are the labia majora (labia = 'lips'; majora = 'larger'), which are larger skin fs olds coated with hair. Nestled within are the labia minora (labia = 'lips'; minora = 'smaller'), which are thinner, more...
31.6K
01:24Disorders of the Female Reproductive System
3.9K
The female reproductive system can be affected by several disorders, including Premenstrual Syndrome (PMS), Premenstrual Dysphoric Disorder (PMDD), endometriosis, and various forms of cancer. PMS and PMDD are cyclical conditions that cause physical and emotional distress, with symptoms that include edema, mood swings, and food cravings. PMDD is a more severe form of PMS characterized by increased symptom severity that peaks during the luteal phase and tends to improve or resolve shortly after...
3.9K
01:20Infection
12.0K
When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...
12.0K
02:43Mouse Models of Cancer Study
6.4K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.4K
01:26Stages of Infection
65.1K
Stages of infection describe what happens to a susceptible host once a pathogen invades the human body. The stages of infection are incubation, prodromal, illness, stage of decline, and convalescence. The incubation stage is the period from exposure to a pathogen until symptoms start. The infected person is unaware of impending illness as the pathogens grow and multiply within the body. The duration may vary depending on the type of infection. The incubation period of measles averages ten to...
65.1K