Analysis of Arginine Metabolism Using LC-MS and Isotopic Labeling
Gretchen L Seim1,2, Emily C Britt1,2, Jing Fan3,4
1Morgridge Institute for Research, Madison, WI, USA.
Abstract:
Arginine metabolism is linked to several important metabolic processes, and reprogramming of arginine metabolism occurs in various physiological and pathological conditions. Here we describe a method, using a LC-MS-based metabolomics and 15N4-arginine tracing approach, to quantitatively analyze arginine metabolism. This method can reliably quantify the abundance of important intermediates and fluxes of major metabolic reactions in arginine metabolism in a variety of cultured mammalian cell models.
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