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Serovars, auxotypes, and plasmid contents of PPNG strains from outbreak in Amsterdam

M C Ansink-Schipper, S M Bygdeman, B van Klingeren

    Genitourinary Medicine
    |June 1, 1987
    PubMed

    Insights

    The first Neisseria gonorrhoeae outbreak in Amsterdam involved African strains with a specific plasmid (Afr+). These strains were non-requiring and inhibited by phenylalanine, with plasmid exchange observed across serovars.

    Area of Science:

    • Microbiology
    • Epidemiology
    • Genetics

    Background:

    • The emergence of penicillinase-producing Neisseria gonorrhoeae (PPNG) poses a significant public health challenge.
    • Understanding the genetic and epidemiological characteristics of PPNG strains is crucial for effective control.

    Purpose of the Study:

    • To characterize the first outbreak of PPNG in Amsterdam (1981-1982).
    • To investigate the genetic makeup and serovar distribution of the causative strains.

    Main Methods:

    • Serological classification using two monoclonal antibody systems.
    • Analysis of plasmid carriage (Afr+) and auxotrophy (non-requiring, NR).
    • Phenotypic characterization including inhibition by phenylalanine (pheni).

    Main Results:

    • The outbreak was primarily caused by African strains carrying the 24-megadalton transfer plasmid (Afr+), which were non-requiring (NR) and phenylalanine-inhibited (pheni).
    • African strains without the transfer plasmid (Afr-) and Afr+ NR strains were also identified.
    • Three main serovars (Ae/Av, Aedih/Arst, Bacejk/Brpyust) were distinguished, indicating plasmid exchanges.
    • Serovar Ae/Av predominated in Afr+ strains, while Bacejk/Brpyust predominated in Afr- strains.

    Conclusions:

    • The Amsterdam PPNG outbreak involved diverse African strains with varying plasmid carriage and auxotrophy.
    • Evidence suggests plasmid exchange occurred within and between serovars.
    • Serovar distribution was associated with plasmid carriage status.

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