Identification of mammalian feces by thin layer chromatography of coprostanol: collaborative study

Mammalian feces contain coprostanol (5 beta-cholestan-3 beta-ol). In this study, 7 collaborators each tested 45 unknown specimens by a thin layer chromatographic method that uses coprostanol as an indicator of feces. The materials tested were 5 replicates each of 3 test portion sizes (0.5, 1.0, and 5.0 mg) of cockroach excreta (negative), and cow and rat feces (both positive). Of 315 specimens tested, 261 (82.9%) were correctly identified; there were 5 false positives, 26 false negatives, and from 1 collaborator, 23 inconclusive results.