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Using autofluorescence to detect bacterial contamination in root fractures
Hye-Min Ku1, Young Ryul Oh2, Eun-Song Lee1
1Department of Preventive Dentistry & Public Oral Health, BK 21 PLUS Project, Yonsei University College of Dentistry, Seoul, South Korea.
Journal of Dentistry
|May 24, 2019
Summary
Autofluorescence imaging detects root fracture depth and bacterial contamination. This advanced technique offers a potential risk indicator for root fractures, surpassing conventional detection methods.
Area of Science:
- Dental Diagnostics
- Biophotonics
- Microbiology
Background:
- Conventional root fracture detection lacks depth and bacterial contamination assessment.
- Root fractures pose significant challenges in endodontically treated teeth.
Purpose of the Study:
- To evaluate autofluorescence for quantifying root fracture depth.
- To determine if autofluorescence can detect bacterial invasion in root fractures.
Main Methods:
- In vitro study of 33 extracted mandibular molars with root fractures.
- Scanning root fractures using a fluorescence technique.
- Analyzing fluorescence parameters against fracture depth and bacterial contamination.
Main Results:
- Fracture light scattering and quantitative fluorescence (ΔFmax) increased with depth.
- Red fluorescence on deeper fractures correlated with bacterial invasion (porphyrins).
- Non-red-fluorescing fractures contained necrotic tissue and debris.
Conclusions:
- Autofluorescence is a viable technique for quantifying root fracture depth.
- This method can serve as a risk indicator for root fractures with periodontal inflammation.
- Autofluorescence shows potential to replace conventional fracture detection methods.
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