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Updated: Jan 24, 2026

Biology of Microbial Communities - Interview
Published on: May 28, 2007
Effect of microbial activity and microbial community structure on the formation of dissolved organic nitrogen (DON)
Kewei Liao1, Haidong Hu1, Sijia Ma1
1State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210023, Jiangsu, PR China.
Abstract:
Dissolved organic nitrogen (DON) formed by microbial metabolism in wastewater treatment processes adversely impacts wastewater reuse and receiving waters quality, and microbial metabolism is greatly influenced by temperatures. However, little is known about the effect of microorganisms on DON and bioavailable DON (ABDON) formation under low temperatures. In this study, six reactors were operated at low (8 °C and 15 °C) and room (25 °C) temperatures to evaluate the relationship between microbial activity, microbial communities, and DON and ABDON. Results showed that DON and ABDON concentrations significantly increased at low temperatures (p < 0.05, t-test). DON formation was significantly correlated to microbial activity only, with adenosine triphosphate (negative, r = -0.64) and polysaccharide (positive, r = 0.61) as key indicators; however, ABDON formation was influenced by both microbial activity (polysaccharide > triphenyltetrazolium chloride-dehydrogenases > adenosine triphosphate) and microbial community structure. Short-term tests using the biomass from six reactors were performed at room temperature to further validate the relationship. The distinct differences between these results provide a basis for different strategies on reducing effluent DON and ABDON under low temperatures.
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