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Related Concept Videos

Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

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Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
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Consider an angioplasty system featuring a catheter equipped with a turbine, a critical tool for removing plaque deposits from coronary arteries. This intricate medical device operates using a circuit model reminiscent of a dual-node RLC circuit powered by a current-controlled voltage source.
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Lymphatic Vessels and Lymph Transport01:16

Lymphatic Vessels and Lymph Transport

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Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
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Fluid Connective Tissues: Blood and Lymph01:20

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Blood and lymph are fluid connective tissues. They contain cells, also known as formed elements, circulating in a liquid extracellular matrix, the plasma. The formed elements are derived from hematopoietic stem cells in the bone marrow. Blood and lymph connect all vital parts and carry nutrients, oxygen, and other essential molecules like antibodies.
Blood
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Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
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Metastasis02:30

Metastasis

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Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
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Related Experiment Video

Updated: Jan 24, 2026

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin
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Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis.

Junqing Hu1, Jinhao Xu1, Mingyue Li1

  • 1State Key Laboratory of Analytical Chemistry for Life Sciences and Collaborative Innovation Center of Chemistry for Life Sciences, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, P.R. China.

Molecular Therapy. Nucleic Acids
|May 24, 2019
PubMed
Summary

A novel cationic agarose (C-agarose) and CpG oligodeoxynucleotide drug delivery system effectively targets lymph nodes. This targeted immunotherapy suppressed tumor metastasis and growth in preclinical models, offering a new approach for cancer treatment.

Keywords:
cancer immunotherapylymph node metastasislymph node sinus macrophages

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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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Intravital Microscopy of the Inguinal Lymph Node
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
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Intravital Microscopy of the Inguinal Lymph Node
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Area of Science:

  • Immunology
  • Oncology
  • Biomedical Engineering

Background:

  • Lymph nodes are crucial immune organs and common sites for cancer metastasis.
  • Effective drug delivery to lymph nodes is essential for blocking cancer spread.
  • Current therapies often lack specificity for lymph node targeting.

Purpose of the Study:

  • To develop a novel drug delivery system for targeted lymph node immunotherapy.
  • To investigate the efficacy of cationic agarose (C-agarose) and CpG oligodeoxynucleotides for blocking lymphatic metastasis.
  • To establish a non-surgical, invasive immunotherapy concept for antitumor metastasis.

Main Methods:

  • Development of a nucleic acid drug delivery system using C-agarose and CpG oligodeoxynucleotides.
  • Utilizing C-agarose's affinity for Siglec-1 on lymph node sinus macrophages for targeted delivery.
  • Subcutaneous implantation of C-agarose+CpG gel in mouse models (4T1 breast cancer, B16F10 melanoma).

Main Results:

  • Achieved targeted accumulation of CpG in lymph node sinus macrophages.
  • Induced significant antitumor immune responses within the lymph node.
  • Demonstrated reduced metastasis size in tumor-draining lymph nodes and lungs.
  • Suppressed tumor growth in both breast cancer and melanoma models.

Conclusions:

  • The C-agarose+CpG gel system provides effective lymph node targeting and immunotherapy.
  • This approach shows promise in reducing cancer metastasis and tumor growth.
  • Proposes a novel, non-surgical strategy for combating antitumor metastasis.