Bioassays and Methodologies for Insecticide Tests with Larvae of Trogoderma granarium (Everts), the Khapra Beetle

New insecticide treatment options would be beneficial for control programs for Trogoderma granarium Everts, the khapra beetle, in the United States. Two insecticides were evaluated, the Polyzone^® formulation of deltamethrin and a formulation of the insect growth regulator methoprene combined with deltamethrin and the synergist piperonyl butoxide. In the test with Polyzone^® deltamethrin, concrete arenas were treated with a low and high rate, and held outside, inside a shed, or inside a lab. Compared to storage in the lab, residue degradation increased slightly in the shed, and then further outside, as evidenced by greater larval survival and adult emergence. Across all environmental treatments, the high rate was more effective than the lower rate. For the combination methoprene product, the effect of food contact with treated surfaces was examined. When treating arenas with food and transferring the food to clean dishes, there was no immediate effect on larval survival, but there was a reduction in survival and emergence to the adult stage after one month. For both tests, larvae apparently often went into diapause after they were introduced onto the treatment arenas. Both treatments could be utilized in management programs if T. granarium infestations are detected.