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Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types
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Application of flow cytometry to immunohematology

S J Nance, G Garratty

    Journal of Immunological Methods
    |July 16, 1987
    PubMed

    A method is described for applying flow cytometry to the analysis of populations of red blood cells that have been sensitized, in vivo or in vitro, with IgG antibody. Specific instrument settings and details of the method are given with sufficient explanation for practical use in immunohematologic investigations.

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    The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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