Related Experiment Video
Updated: Jan 24, 2026

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
Published on: April 17, 2017
Phthalocyanine-doped polystyrene fluorescent nanocomposite as a highly selective biosensor for quantitative
M S Attia1, K Ali2, Maged El-Kemary3
1Chemistry Department, Faculty of Science, University of Jeddah, P.O. Box 80327, Jeddah, 21589, Saudi Arabia; Chemistry Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt.
Abstract:
A novel, simple, sensitive, and precise spectrofluorometric assay of cancer antigen [CA 125] is described. This modality is based on monitoring the quenching of the luminescence intensity at 790 nm of the phthalocyanine fluorophore, in a nanocomposite comprising the fluorophore and cationic polystyrene, which results from interaction with CA 125. The remarkable quenching of the luminescence intensity of the Ni-phthalocyanine complex doped in PS matrix by various concentrations of CA 125 was successfully utilized as an optical sensor for the determination of CA 125 in different serum samples of ovarian disease. The performance of the designed biosensor is determined through monitoring the quenching of the luminescence intensity at 790 nm by cancer antigen 125 after excitation at 685 nm, pH 7.3 in water. The calibration plot was achieved over the concentration range 1.0 × 10-2 - 127 U mL-1 CA-125 with a correlation coefficient of 0.99 and detection limit of 1.0 × 10-4 U mL-1. The mechanism of the interaction between the nano thin film nickel(II)phthalocyanine and CA-125 was discussed. A significant correlation between the proposed method for the assessment of CA 125 and the standard method was applied to patients and controls.
More Related Videos
08:27Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
09:53Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
Published on: February 6, 2017
Related Concept Videos
What is Natural Selection?
Antibiotic Selection
Types of Selection
Antigen Presenting Cells
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
Frequency-dependent Selection
Determining Order of Reaction