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Updated: Jan 24, 2026

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
Published on: November 29, 2016
A near-infrared fluorescent probe for imaging endogenous carbon monoxide in living systems with a large Stokes shift
Shengyi Gong1, Jiaxin Hong1, Enbo Zhou1
1Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, PR China.
Abstract:
Near-infrared (NIR) fluorescent probes with a large Stokes shift are very practical tools for bioimaging applications. Carbon monoxide (CO) is a key gaseous signal molecule and its imaging in living systems has attracted great attention in recent years. In this work, a very easy-to-get NIR fluorescent probe with a remarkable large pseudo-Stokes shift (238 nm) for detection of CO was reported. This probe was found to show a rapid NIR fluorescent turn-on response for CO with high selectivity, high sensitivity and a low detection limit (38 nM). Moreover, imaging CO in living cells and animals with this probe was successfully applied with a high signal-to-noise ratio. The results indicate that this probe can be used as a new practical tool for imaging of endogenous CO in living systems.
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