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Related Experiment Video

Updated: Jan 24, 2026

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Simple and Fast Rolling Circle Amplification-Based Detection of Topoisomerase 1 Activity in Crude Biological Samples

Published on: December 2, 2022

Simple rolling circle amplification colorimetric assay based on pH for target DNA detection.

Seyed Vahid Hamidi¹, Jonathan Perreault¹

¹INRS, Centre INRS - Institut Armand-Frappier, 531 Boul. des Prairies, Laval, Québec, Canada.

|May 25, 2019

Summary

This study introduces a novel colorimetric assay for DNA detection using PadLock Probe (PLP)-based Rolling Circle Amplification (RCA). The portable assay detects low picomolar DNA quantities in 20 minutes without specialized equipment.

Keywords:

Biosensor Bst DNA polymerase Colorimetric assay Padlock probe Rolling circle amplification Unbuffered ligation

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Published on: June 9, 2011

Area of Science:

Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Polymerase Chain Reaction (PCR) enables sensitive DNA detection but requires laboratory equipment.
Isothermal amplification techniques offer alternatives to PCR, but often still need specialized apparatus.
PadLock Probe (PLP)-based Rolling Circle Amplification (RCA) is an isothermal method with potential for simplified detection.

Purpose of the Study:

To develop a portable, equipment-free colorimetric assay for DNA detection.
To leverage PLP-based RCA for sensitive DNA identification.
To enable visual DNA detection without thermocyclers or complex analytical devices.

Main Methods:

Development of a colorimetric assay based on pH change.
Integration of the assay with PadLock Probe (PLP)-based Rolling Circle Amplification (RCA).
Assessment of DNA detection limits and time requirements.

Main Results:

The assay successfully detects DNA in the low picomolar range.
Results are observable to the naked eye within 20 minutes.
The method eliminates the need for thermocyclers and other complex laboratory equipment.

Conclusions:

A novel, portable, and visually detectable colorimetric DNA assay has been developed.
This assay utilizes PLP-based RCA and a pH-change indicator for simplified DNA detection.
The technology offers a promising solution for field-based or resource-limited DNA analysis.