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Updated: Jan 24, 2026

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Complete Genome Sequence of Klebsiella pneumoniae Podophage Patroon

Rainie Tran1, Rohit Kongari1, Lauren Lessor1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Microbiology Resource Announcements
|May 25, 2019
PubMed

Insights

Multidrug-resistant Klebsiella pneumoniae infections are a growing hospital threat. Studying K. pneumoniae phages, like the newly sequenced phage Patroon, offers potential for novel therapeutic approaches against these resistant bacteria.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Klebsiella pneumoniae infections pose a significant threat in healthcare environments.
  • The rise of multidrug-resistant (MDR) strains complicates treatment options.
  • Bacteriophages (phages) targeting K. pneumoniae are being investigated as alternative therapies.

Purpose of the Study:

  • To present the complete genome sequence of Klebsiella pneumoniae phage Patroon.
  • To characterize phage Patroon as a T3/T7-like phage.
  • To contribute genomic data for the development of phage-based therapies against K. pneumoniae.

Main Methods:

  • Isolation and purification of Klebsiella pneumoniae phage Patroon.
  • Whole-genome sequencing of the phage.
  • Bioinformatic analysis of the phage genome.

Main Results:

  • The complete genome sequence of K. pneumoniae phage Patroon was successfully obtained.
  • Phage Patroon was identified as belonging to the T3/T7-like phage group.
  • Genomic data provides a foundation for understanding phage-host interactions.

Conclusions:

  • The genomic information of phage Patroon is valuable for K. pneumoniae research.
  • This study supports the potential of bacteriophage therapy in combating MDR K. pneumoniae infections.
  • Further research into K. pneumoniae phages can lead to innovative treatment strategies.

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