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Related Experiment Video

Updated: Jan 24, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Kidney Cortical Transporter Expression across Species Using Quantitative Proteomics.

Abdul Basit¹, Zaher Radi¹, Vishal S Vaidya¹

¹Department of Pharmaceutics, University of Washington, Seattle, Washington (A.B., M.K., B.P.); and Pfizer Worldwide Research Development, Drug Safety Research Development, Cambridge, Massachusetts (Z.R., V.S.V.).

Drug Metabolism and Disposition: the Biological Fate of Chemicals

|May 25, 2019

Summary

Species differences in kidney transporter abundance impact drug safety. Preclinical species generally show higher transporter levels than humans, affecting drug clearance predictions and toxicity assessments.

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Published on: October 19, 2021

Area of Science:

Pharmacology
Nephrology
Drug Development

Background:

Understanding species-specific kidney transporter expression is crucial for predicting drug pharmacokinetics, interactions, and toxicity.
Existing knowledge gaps hinder accurate translation of preclinical drug data to human outcomes, particularly for acute kidney injury.

Purpose of the Study:

To quantify and compare the protein abundance of 19 key kidney transporters across five species: human, monkey, dog, rat, and mouse.
To identify cross-species and sex-dependent variations in transporter expression relevant to drug development.

Main Methods:

Quantitative analysis of protein abundance for 19 membrane transporters in kidney cortex samples from human, monkey, dog, rat, and mouse.
Statistical analysis to determine significant differences in expression levels and identify correlations between transporters.

Main Results:

Most transporters were more abundant in preclinical species than in humans, with notable exceptions like MDR1, OCT3, and OCTN1.
Significant cross-species variability was observed for transporters including MRP4, OCTN2, OAT2, and OCT2.
Sex differences in transporter abundance were primarily observed in rodents and dogs.

Conclusions:

Kidney transporter abundance varies significantly across species and, in some cases, by sex, necessitating careful consideration during drug development.
These data are vital for improving animal-to-human drug clearance scaling and for assessing the potential kidney toxicity of new drug candidates.