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Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
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Long noncoding RNA UBE2R2-AS1 promotes glioma cell apoptosis via targeting the miR-877-3p/TLR4 axis

Wu Xu1, Guo-Qing Hu1, Clive Da Costa2

  • 1Department of Epidemiology and Health Statistics, Xiangya School of Public Health, Central South University, Changsha 410078, People's Republic of China.

Oncotargets and Therapy
|May 25, 2019
PubMed

Insights

A novel long noncoding RNA, UBE2R2-AS1, is downregulated in brain glioma. UBE2R2-AS1 suppresses glioblastoma growth by targeting miR-877-3p and Toll-like receptor 4 (TLR4).

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Brain glioma, particularly glioblastoma (GBM), is a prevalent and aggressive CNS malignancy.
  • Long noncoding RNAs (lncRNAs) are implicated in cancer but their specific roles in glioma require further elucidation.
  • Understanding lncRNA mechanisms is crucial for developing novel glioma therapies.

Purpose of the Study:

  • To identify and characterize novel lncRNAs involved in glioma pathogenesis.
  • To investigate the molecular mechanisms of UBE2R2-AS1 in glioma.
  • To explore the potential of UBE2R2-AS1 as a therapeutic target.

Main Methods:

  • Microarray analysis of glioma and adjacent normal tissues to identify differentially expressed lncRNAs.
  • In vitro experiments to assess the functional impact of UBE2R2-AS1 on glioma cell behavior.
  • Investigation of the competing endogenous RNA (ceRNA) network involving UBE2R2-AS1, miR-877-3p, and TLR4.

Main Results:

  • UBE2R2-AS1 was significantly downregulated in glioma tissues compared to normal tissues.
  • UBE2R2-AS1 overexpression inhibited glioma cell proliferation, migration, and invasion.
  • UBE2R2-AS1 functions as a ceRNA, targeting miR-877-3p to regulate Toll-like receptor 4 (TLR4) expression and glioblastoma progression.
  • UBE2R2-AS1 promoted glioma cell apoptosis.

Conclusions:

  • UBE2R2-AS1 acts as a tumor suppressor in glioma.
  • The UBE2R2-AS1/miR-877-3p/TLR4 axis represents a novel molecular mechanism in glioblastoma.
  • UBE2R2-AS1 holds potential for future glioma diagnostics and targeted therapies.
Keywords:
UBE2R2-AS1apoptosisbrain gliomalncRNAmiR-877-3p/TLR4

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