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Related Experiment Video

Updated: Jan 24, 2026

Isolation of microRNAs from Tick Ex Vivo Salivary Gland Cultures and Extracellular Vesicles
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Extracellular vesicle microRNA quantification from plasma using an integrated microfluidic device.

Zeinab Ramshani1,2,3, Chenguang Zhang1,2, Katherine Richards3,4

  • 11Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556 USA.

Communications Biology
|May 25, 2019
PubMed
Summary

We developed a rapid, PCR-free microfluidics platform for quantifying microRNAs (miRNAs) in extracellular vesicles (EVs). This technology offers sensitive and quick disease biomarker detection using minimal plasma.

Keywords:
Lab-on-a-chipRNA probesmiRNAs

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Biomarker Discovery

Background:

  • Extracellular vesicles (EVs) carrying microRNAs (miRNAs) show promise as early disease biomarkers.
  • Current methods for miRNA quantification are often time-consuming and require large sample volumes.

Purpose of the Study:

  • To develop a simple, rapid, and sensitive PCR-free platform for absolute quantification of free-floating and EV-miRNAs.
  • To enable early disease detection and monitoring using minimal patient samples.

Main Methods:

  • An integrated microfluidics platform combining surface acoustic wave (SAW) EV lysis with electrokinetic membrane sensing.
  • Absolute quantification of miRNAs without RNA extraction, purification, reverse transcription, or amplification.
  • Utilizes non-equilibrium ionic currents for sensitive detection.

Main Results:

  • Achieved absolute quantification of miRNAs with <10% uncertainty and 1 pM detection sensitivity.
  • Assay time reduced to 30 minutes from conventional 13 hours.
  • Requires only ~20 μL of plasma, a significant reduction from 1 mL.

Conclusions:

  • The developed platform offers a rapid, sensitive, and efficient method for miRNA quantification.
  • It eliminates the need for complex pre-processing steps associated with conventional techniques.
  • This technology serves as a viable screening tool for early disease diagnosis, prognosis, and therapeutic response monitoring.