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Updated: Jan 24, 2026

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
Published on: May 5, 2009
High-Content Autophagy Analysis in iPSC-Derived Neurons Using Immunofluorescence
Daniel Little1, Christin Luft2, Olukunbi Mosaku2
1MRC Laboratory for Molecular Cell Biology, University College London, London, UK. d.little@ucl.ac.uk.
Abstract:
Autophagy is the process by which cellular proteins and organelles are degraded and recycled and is essential to the survival of cells. Defective autophagic degradation has been linked to many neurodegenerative diseases and in particular lysosomal storage diseases. Here we describe a high-content assay to detect defects in the autophagy pathway in induced pluripotent stem cell-derived neurons. This assay utilizes immunofluorescence to stain autophagosomes and uses automated image analysis to measure changes in autophagosome levels in response to modulators of autophagy.
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