Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Elongation Factors02:35

Transcription Elongation Factors

13.5K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.5K
Transcription Elongation Factors02:35

Transcription Elongation Factors

4.6K
4.6K
Transcription Factors02:16

Transcription Factors

82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.6K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.6K
General Transcription Factors01:30

General Transcription Factors

6.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.2K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nucleoid-associated protein HU constrains transcription elongation to influence cellular functions, virulence, and drug tolerance in Mycobacterium tuberculosis.

Nucleic acids research·2026
Same author

Correction: Genomic islands and plasmid borne antimicrobial resistance genes drive the evolution of high-risk, ST-131 uropathogenic E. coli NS30.

BMC genomics·2026
Same author

Genomic islands and plasmid borne antimicrobial resistance genes drive the evolution of high-risk, ST-131 uropathogenic E. coli NS30.

BMC genomics·2025
Same author

Succinylation of GyrB Is a Mechanism Conferring Fluoroquinolone Resistance.

ACS infectious diseases·2025
Same author

Inhibition of DNA cleavage and strand passage activities of Mycobacterium tuberculosis topoisomerase I.

Nucleic acids research·2025
Same author

Biophysical, sequence and structural analysis of type 3 secretion system pilus protein HrpA of <i>Pseudomonas syringae</i>: insights into HrpA pili stability and assembly.

Journal of biomolecular structure & dynamics·2025

Related Experiment Video

Updated: Jan 24, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.0K

Mycobacterial transcript cleavage factor Gre, exhibits chaperone-like activity.

Abyson Joseph1, Valakunja Nagaraja2, Ramanathan Natesh1

  • 1School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram, Maruthamala P.O., Vithura, Trivandrum, Kerala 695551, India.

Biochimica Et Biophysica Acta. Proteins and Proteomics
|May 25, 2019
PubMed
Summary
This summary is machine-generated.

Mycobacterium smegmatis Gre (MsGre) factor exhibits moonlighting chaperone activity, preventing protein aggregation and preserving enzyme function under heat stress. This essential protein maintains its structure and function, highlighting its crucial role in cellular protection.

More Related Videos

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

14.0K
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
08:32

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Published on: October 23, 2016

11.0K

Related Experiment Videos

Last Updated: Jan 24, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

15.0K
Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

14.0K
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
08:32

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Published on: October 23, 2016

11.0K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Protein Chemistry

Background:

  • Gre factors regulate transcription by enhancing RNA polymerase cleavage activity.
  • Mycobacterium tuberculosis Gre factor is essential for survival, unlike in E. coli.
  • Gre factors are implicated in stress response, with E. coli GreA acting as a chaperone.

Purpose of the Study:

  • To characterize the moonlighting chaperone activity of Mycobacterium smegmatis Gre (MsGre).
  • To elucidate the mechanism behind MsGre's chaperone function under thermal stress.

Main Methods:

  • Assessed MsGre's ability to prevent heat-induced protein aggregation and protect enzymatic activity.
  • Utilized 8-anilino-1-naphthalene sulfonate (ANS) binding assays to detect hydrophobic surface exposure.
  • Employed Circular Dichroism (CD) spectroscopy to evaluate secondary structure stability under thermal stress.

Main Results:

  • MsGre prevents heat-induced aggregation of substrate proteins and preserves their enzymatic activity.
  • MsGre exists as a dimer and does not oligomerize upon heat stress.
  • ANS binding indicates MsGre exposes hydrophobic surfaces upon heat stress, facilitating substrate interaction.
  • CD studies confirm MsGre maintains a stable secondary structure under thermal stress.

Conclusions:

  • MsGre possesses significant moonlighting chaperone activity, crucial for cellular protection against heat stress.
  • The C-terminal FKBP-like fold in MsGre is proposed to contribute to its chaperone function.
  • MsGre's dual role in transcription regulation and stress response underscores its importance in Mycobacterium survival.