Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mutagen detection with a mouse line containing 3 distinct mutations conferring sensitivity.

T Shiomi, N Hieda-Shiomi, H Hama-Inaba

    Mutation Research
    |August 1, 1987
    PubMed
    Summary

    A new mouse cell mutant, XUM1, shows high sensitivity to various mutagens including methyl methanesulfonate (MMS), X-rays, and UV light. This characteristic makes XUM1 cells valuable for detecting a broad spectrum of mutagens and carcinogens.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Macrophages in protective immunity to Hymenolepis nana in mice.

    Immunology and cell biology·1992
    Same author

    Establishment of a folliculo-stellate-like cell line from a murine thyrotropic pituitary tumor.

    Endocrinology·1992
    Same author

    The effects of sodium chloride, miso or ethanol on development of intestinal metaplasia after X-irradiation of the rat glandular stomach.

    Japanese journal of cancer research : Gann·1992
    Same author

    Biochemical characterization of programmed cell death in NGF-deprived sympathetic neurons.

    Journal of neurobiology·1992
    Same author

    The carboxy-terminal 10 amino acid residues of cytochrome b5 are necessary for its targeting to the endoplasmic reticulum.

    The EMBO journal·1992
    Same author

    Chemotactic factor in the pregnant rabbit uterine cervix.

    American journal of obstetrics and gynecology·1992

    Area of Science:

    • Genetics and Molecular Biology
    • Mutagenesis and Carcinogenesis Research

    Background:

    • Development of sensitive assays for mutagens and carcinogens is crucial for public health.
    • Existing cell-based assays have limitations in detecting a wide range of genotoxic agents.

    Purpose of the Study:

    • To select and characterize a novel multiple mutagen-sensitive cell mutant.
    • To evaluate the utility of this mutant for sensitive detection of mutagens and carcinogens.

    Main Methods:

    • Selection of a mouse cell mutant (XUM1) using replica plating and cell suspension spotting.
    • Assessment of sensitivity to various DNA-damaging agents including methyl methanesulfonate (MMS), X-rays, ultraviolet light (UV), mitomycin C, and 4-nitroquinoline-1-oxide (4NQO).
    • Growth inhibition area tests to determine mutagen detection sensitivity.

    Related Experiment Videos

    Main Results:

    • A new mutant, XUM1, derived from XU1, was identified with hypersensitivity to MMS, X-rays, UV, mitomycin C, and 4NQO.
    • XUM1 demonstrated the ability to detect low concentrations of multiple mutagens.
    • The mutant exhibited enhanced sensitivity compared to previously characterized cell lines.

    Conclusions:

    • XUM1 represents a highly sensitive tool for detecting a wide array of mutagens and carcinogens.
    • This mutant can be utilized in developing advanced screening assays for genotoxic substances.
    • XUM1 offers a valuable resource for environmental monitoring and toxicological studies.