Evaluation of split-time artificial insemination following administration of a long or short-term progestin-based...

Fixed-time and split-time AI were compared following the melengestrol acetate (MGA^®) prostaglandin F_2α (Experiment 1) and 7-d CO-Synch + controlled internal drug release (CIDR^®) protocols (Experiment 2). Heifers in Experiments 1 (n = 524) and 2 (n = 456) were assigned within pen to balanced treatments based on weight and reproductive tract score (RTS; Scale 1-5). In Experiment 1, MGA^® (0.5 mg∙animal^-1∙d^-1) was fed for 14 d, and prostaglandin F_2α (PG; 250 μg im cloprostenol sodium) was administered 19 d after MGA^® withdrawal. In Experiment 2, gonadotropin-releasing hormone (GnRH; 100 μg gonadorelin acetate) was administered coincident with CIDR^® (1.38 g progesterone [P₄]) insertion. Inserts were removed after 7 d, and PG (250 μg im cloprostenol sodium) was administered at CIDR^® removal. In both experiments, estrus detection aids (Estrotect^®) were applied at the time of PG administration. Estrous status was recorded at FTAI or STAI. Estrus was defined as removal of ≥ 50% of the grey coating from the Estrotect^® patch. Heifers assigned to FTAI treatments received GnRH and were artificially inseminated at the standard time for FTAI for each protocol: 72 or 54 h after PG administration for the MGA-PG or 7-d CO-Synch + CIDR^® protocol, respectively. In the STAI treatments, only heifers that expressed estrus prior to the standard time of FTAI were artificially inseminated at that time. For heifers failing to express estrus, AI was postponed 24 h. Only heifers that failed to exhibit estrus by the delayed time received GnRH concurrent with AI. In both experiments, estrous response prior to the standard time of FTAI did not differ between treatments. Total estrous response was increased (P < 0.01) among heifers assigned to STAI in Experiment 1 (88%, STAI; 72%, FTAI) and 2 (74%, STAI; 47%, FTAI). In Experiment 1, pregnancy rates resulting from AI were greater (P < 0.04) for heifers assigned to STAI compared with FTAI (55% vs 46%, respectively). In Experiment 2, pregnancy rates resulting from AI were similar between treatments (48% and 46%, respectively; P = 0.6). In summary, when compared with FTAI, STAI resulted in greater estrous response following both the MGA^®-PG and 7-d CO-Synch + CIDR^® protocols. The increased estrous response through use of STAI was associated with a corresponding increase in pregnancy rates to AI following the MGA^®-PG protocol; however, a similar improvement in pregnancy rates was not observed following the 7-d CO-Synch + CIDR^® protocol.