Related Experiment Video

Updated: Jan 24, 2026

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

9.5K

Anticancer saponin OSW-1 is a novel class of selective Golgi stress inducer

Mayu Kimura1, Kanae Sasaki2, Yosuke Fukutani1

  • 1Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-18, Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.

Bioorganic & Medicinal Chemistry Letters
|May 26, 2019
PubMed

Insights

OSW-1, a natural compound, kills cancer cells by activating Golgi stress, leading to programmed cell death. This study reveals the mechanism behind its anticancer effects by targeting lipid homeostasis.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • OSW-1 is a plant-derived natural product with proposed anticancer properties.
  • Its mechanism involves binding to oxysterol binding protein family members, disrupting lipid homeostasis.
  • The precise signaling pathways mediating OSW-1-induced cell death were previously unknown.

Purpose of the Study:

  • To elucidate the molecular mechanism by which OSW-1 induces cancer cell death.
  • To investigate the role of Golgi stress response in OSW-1's anticancer activity.

Main Methods:

  • Cell-based assays to assess apoptosis and Golgi stress.
  • Biochemical analyses to study protein-ligand interactions.
  • Lipidomics to evaluate homeostasis disruption.

Main Results:

  • OSW-1 selectively activates the Golgi stress response in cancer cells.
  • Activation of Golgi stress by OSW-1 leads to apoptosis.
  • The findings provide a mechanistic link between lipid homeostasis disruption and cell death signaling.

Conclusions:

  • OSW-1's anticancer activity is mediated by the activation of the Golgi stress response.
  • This study provides a mechanistic basis for OSW-1's therapeutic potential.
  • Targeting Golgi stress represents a novel strategy for cancer therapy.
Keywords:
Anticancer natural productGolgi stress responseOSBPOSW-1Saponin

More Related Videos

The Trier Social Stress Test Protocol for Inducing Psychological Stress09:39

The Trier Social Stress Test Protocol for Inducing Psychological Stress

Published on: October 19, 2011

58.1K
Restraint to Induce Stress in Mice and Rats03:48

Restraint to Induce Stress in Mice and Rats

Published on: December 6, 2024

3.2K

Related Concept Videos

Golgi Apparatus01:49

Golgi Apparatus

As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
101.0K
Golgi Apparatus01:09

Golgi Apparatus

Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
21.2K
Transport Across the Golgi01:26

Transport Across the Golgi

While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
6.0K
Golgi Matrix Proteins01:12

Golgi Matrix Proteins

Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
2.4K
Drug Classes and Categories01:25

Drug Classes and Categories

Drugs can be classified according to their chemical composition or their intended therapeutic application. For instance, anti-infective agents that possess the ability to eliminate pathogens or suppress their growth and reproduction can be grouped based on the organisms they target or their chemical structure. Furthermore, drugs can be divided into prescription, nonprescription, or controlled substances. Prescription medications, such as antibiotics, require oversight from a licensed healthcare...
3.1K
Antibody Structure and Classes01:25

Antibody Structure and Classes

Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
8.4K