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Related Experiment Video

Updated: Jan 24, 2026

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy
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Multifunctional graphene supports for electron cryomicroscopy.

Katerina Naydenova1, Mathew J Peet1, Christopher J Russo2

  • 1Medical Research Council Laboratory of Molecular Biology, Cambridge, CB2 0QH, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|May 26, 2019
PubMed
Summary

Researchers developed a new multifunctional specimen support for electron cryomicroscopy (cryoEM) using graphene and gold. This novel support improves specimen stability and image quality, enabling high-resolution biomolecular structure determination with less material and data.

Keywords:
cryoEMgraphene functionalizationlow-energy plasmastructure determination

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Area of Science:

  • Structural biology
  • Biophysics
  • Materials science

Background:

  • Single-particle electron cryomicroscopy (cryoEM) is a powerful technique for determining atomic resolution structures of biomolecular complexes.
  • Specimen quality, specifically proper vitrification, is a critical bottleneck for high-resolution cryoEM.
  • Current methods often require significant amounts of purified sample and can suffer from specimen drift during imaging.

Purpose of the Study:

  • To develop an improved specimen support for cryoEM that enhances sample stability and image quality.
  • To reduce the amount of material and data required for high-resolution structure determination.
  • To create a versatile support adaptable to various biomolecular specimens.

Main Methods:

  • Fabrication of a multifunctional specimen support using large-crystal monolayer graphene on an ultrastable gold grid.
  • Utilizing a low-energy plasma system for surface modification and covalent functionalization of the graphene layer.
  • Testing the support's performance in single-particle cryoEM imaging and structure determination.

Main Results:

  • The novel graphene-gold support significantly reduces specimen movement during cryoEM imaging.
  • Improved image quality was observed, facilitating higher resolution data collection.
  • Successful high-resolution structure determination was achieved with minimal sample and data requirements.

Conclusions:

  • The multifunctional specimen support represents a significant advancement for cryoEM sample preparation.
  • This technology lowers the barrier to high-resolution structure determination, making it more accessible.
  • The tunable surface properties offer broad applicability for various biomolecular complexes.