Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alkali Metals03:06

Alkali Metals

24.3K
Group 1 elements are soft and shiny metallic solids. They are malleable, ductile, and good conductors of heat and electricity. The melting points of the alkali metals are unusually low for metals and decrease going down the group, while the density increases going down the group with the exception of potassium (Table 1).
Table 1: Properties of the alkali metals
24.3K
Bonding in Metals02:32

Bonding in Metals

52.2K
Metallic bonds are formed between two metal atoms. A simplified model to describe metallic bonding has been developed by Paul Drüde called the “Electron Sea Model”. 
52.2K
Metallic Solids02:37

Metallic Solids

20.5K
Metallic solids such as crystals of copper, aluminum, and iron are formed by metal atoms. The structure of metallic crystals is often described as a uniform distribution of atomic nuclei within a “sea” of delocalized electrons. The atoms within such a metallic solid are held together by a unique force known as metallic bonding that gives rise to many useful and varied bulk properties.
All metallic solids exhibit high thermal and electrical conductivity, metallic luster, and malleability....
20.5K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

24.1K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
24.1K
Green Algae01:21

Green Algae

756
Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
756
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

1.8K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Competing programs shape cortical sensorimotor-association axis development.

Nature·2026
Same author

Bonding character and octahedral geometry as key determinants of solute miscibility in Ir-based oxides.

Nature communications·2026
Same author

Post-Synthetic Modification of the C═C Linkage in a Vinylene-Linked COF for ROS-Mediated Enamine Dimerization.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Polyketide synthase-like functionality acquired by plant fatty acid elongase.

Science advances·2026
Same author

scDecorr: feature decorrelation based representation learning enables self-supervised alignment of multiple single-cell experiments.

Scientific reports·2026
Same author

Impact of Transfusion Dependence on Clinical and Economic Burden in Patients with Lower-Risk Myelodysplastic Syndromes: A 28-Year Retrospective Study.

Journal of blood medicine·2026

Related Experiment Video

Updated: Jan 24, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K

Heavy metal(loid) biosensor based on split-enhanced green fluorescent protein: development and characterization.

Hyojin Kim1, Woonwoo Lee1, Youngdae Yoon2

  • 1Department of Environmental Health Science, Konkuk University, Seoul, 05029, South Korea.

Applied Microbiology and Biotechnology
|May 26, 2019
PubMed
Summary

Researchers developed a novel biosensor using enhanced green fluorescent protein (eGFP) to detect heavy metals like cadmium (Cd) and mercury (Hg). This practical alternative offers a new approach for environmental monitoring of toxic metal(loid)s.

Keywords:
BiosensorsCadmiumEnhanced green fluorescent proteinHeavy metalMercuryMetal-binding loop (MBL)

More Related Videos

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection
07:25

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

11.1K
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
10:20

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae

Published on: July 10, 2015

16.4K

Related Experiment Videos

Last Updated: Jan 24, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K
Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection
07:25

Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

Published on: May 24, 2018

11.1K
Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
10:20

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae

Published on: July 10, 2015

16.4K

Area of Science:

  • Biochemistry
  • Environmental Science
  • Biosensor Technology

Background:

  • Heavy metal(loid)s like cadmium (Cd) and mercury (Hg) pose significant risks to human health and the environment.
  • Current methods for detecting these metals often rely on expensive and time-consuming instrumental analyses.
  • There is a need for practical and accessible alternatives for monitoring heavy metal(loid) contamination.

Purpose of the Study:

  • To engineer enhanced green fluorescent protein (eGFP)-based biosensors for the detection of heavy metal(loid)s.
  • To demonstrate that metal-binding loops (MBLs) inserted into eGFP can restore fluorescence upon metal ion binding.
  • To develop a practical platform for quantifying Cd and Hg in environmental samples.

Main Methods:

  • Engineered eGFP by inserting metal-binding loops (MBLs) into a specific loop region, rendering the protein inactive.
  • Observed restoration of fluorescence in the engineered eGFP upon binding of Cd and Hg ions.
  • Quantified metal concentrations within a specific range (0-5 μM) using the fluorescence signal.
  • Validated the sensing platform by measuring Cd in artificially contaminated soil and water samples.

Main Results:

  • The engineered eGFP regained fluorescence upon exposure to Cd and Hg, indicating successful metal binding.
  • The biosensor demonstrated the ability to quantify Cd and Hg in the concentration range of 0-5 μM.
  • Initial tests in artificially amended soil and water samples showed potential for practical application, though sensor performance requires improvement.

Conclusions:

  • The developed eGFP-based biosensor offers a novel approach for heavy metal(loid) detection.
  • The design concept of using MBLs to modulate eGFP fluorescence is valuable for heavy metal sensing.
  • Further improvements are needed to enhance sensor performance for widespread environmental monitoring applications.