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Updated: Jan 24, 2026

Methods for Experimental Manipulations after Optic Nerve Transection in the Mammalian CNS
Published on: May 12, 2011
Analyses of Cholesterol Metabolites of Optic Nerve Using GC-MS Methods
Gurkaran S Sarohia1, Erik D Garza2, Sanjoy K Bhattacharya3
1MD Undergraduate Program, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. Gurkaran.Sarohia@alumni.ubc.ca.
Abstract:
Gas chromatography-mass spectrometry (GC-MS) is considered the gold standard for analyzing and quantifying the presence of biological compounds in tissue samples due to its high sensitivity, peak resolution, and reproducibility. In this chapter, we describe a step-by-step modified Bligh and Dyer protocol for lipid extraction from the optic nerve tissue and a procedure for GC-MS analyses of the lipid extract. These protocols are based on our experience and can be modified depending on samples and compounds of interest.
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