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Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry
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HSD18B7 Enzyme Assay Technique Using a Triple Quadrupole Mass Spectrometer

Carina Wu1, Nhuong-Sao Ton1, Maria Fernanda Suarez2

  • 1Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2019
PubMed
Summary

This study investigated the enzyme HSD17B7 in glaucoma. We found altered HSD17B7 activity in glaucomatous optic nerves, suggesting a role for cholesterol biosynthesis in the disease.

Keywords:
Biosynthesis pathwayCatalyzeCholesterolDihydrotestosterone (DHT)HSD17B7 enzymeSubstrateTriple quadrupole mass spectrometer

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