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Updated: Jan 24, 2026
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Retinal Vascular Reactivity as Assessed by Optical Coherence Tomography Angiography
Published on: March 26, 2020
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Metabolomics Applicable to Retinal Vascular Diseases
Satish Patnaik1, Subhadra Jalali2, Manjunath B Joshi3
1Kallam Anji Reddy Molecular Genetics Laboratory, Prof. Brien Holden Eye Research Centre, L V Prasad Eye Institute, Hyderabad, India.
Methods in Molecular Biology (Clifton, N.J.)
|May 26, 2019
Summary
Vitreous humor analysis offers a viable alternative to retinal tissue for metabolomic studies in retinal vascular diseases. This approach aids in understanding metabolic disruptions in conditions like age-related macular degeneration and diabetic retinopathy.
Area of Science:
- Metabolomics and its application in understanding biological systems.
- Biochemical analysis of human ocular fluids.
Background:
- The human retina is metabolically active, and its metabolic activity is often disrupted in various vascular diseases.
- Studying retinal metabolites directly is challenging due to the limited availability of diseased human retinal tissue.
- Vitreous humor, being in close proximity to the retina, can serve as a surrogate for retinal metabolomic analysis.
Purpose of the Study:
- To explore the potential of vitreous humor as a surrogate for retinal metabolomic profiling.
- To compare different solvent-based extraction methods for analyzing metabolites in vitreous humor from patients with retinal vascular diseases.
- To identify and quantify metabolites in vitreous humor using LC-MS/MS.
Main Methods:
- Vitreous humor samples were collected from patients with retinal vascular diseases.
- Two distinct solvent-based extraction methods (methanol and chloroform) were employed to extract metabolites.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilized for the detection and identification of extracted metabolites.
Main Results:
- The study successfully extracted and analyzed metabolites from human vitreous humor using two different solvent-based methods.
- LC-MS/MS enabled the detection and identification of a range of metabolites present in the vitreous humor samples.
- The findings suggest that vitreous humor can be effectively used for metabolomic profiling relevant to retinal vascular diseases.
Conclusions:
- Vitreous humor metabolomics is a feasible approach for studying metabolic alterations in retinal vascular diseases.
- The choice of extraction solvent impacts the profile of detectable metabolites.
- This surrogate approach facilitates the investigation of retinal metabolic pathways disrupted in diseases like age-related macular degeneration and diabetic retinopathy.
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