[Purification of alkaline proteinase from Bacillus subtilis 72 using ion-exchange chromatography]

Alkaline proteinase was isolated from the culture fluid filtrate obtained as a result of cultivation of Bacillus subtilis st. 72 using ion-exchange chromatography on a modified silochrome. Sorption of the enzyme at pH 6.0-6.3 and desorption at pH 7.5-7.8 followed by ultrafiltration through the membrane UAM-150 (USSR) resulted in a 5-fold increase of the enzyme specific activity calculated in respect of protein and an activity yield of 84%.